:Normal range
Hb A1 : 95-98%
Hb A2 : 1.5-3.5%
Hb F : up to 2.0%
Hb S : Absent
Hb C : Absent
Comment: Electrophoresis for separation and distribution of hemoglobins (Hb A1), fetal hemoglobin (Hb F) and hemoglobin A2 (Hb A2) often by other methods, in particular column or high performance liquid chromatography. In this procedure, hemoglobins are caused to separate and migrate. A variety of techniques are utilized. Most common hemoglobins are separated as discrete bands as they move through a substrate in a buffer solution across an electric field with subsequent visualization by fixation and staining (hemoglobin electrophoresis). Clinical applications include detection and identification of hemoglobin variants and the investigation of some hemolytic anemias resulting from red cell intra corpuscular defects. Use to diagnose hemoglobinpathies; evaluate sickling hemoglobins, hemoglobin C; with other and specialized techniques, evaluate unstable, low and high oxygen affinity hemoglobinopathies the latter representing one cause of polycythemia
Sample: 5 ml, EDTA, whole blood